Interaction between the cellular mRNA and the cellular microRNA linked to human immunodeficiency virus latency
Neil Tan Gana, Okamoto T.
Clin Vaccine Immunol. 2013(in press)

ABSTRACT: Although microRNAs (miR/s) play regulatory roles in a wide variety of cellular events, the regulatory mechanisms of miRs associated with viral diseases such as human immunodeficiency virus (HIV-1) infection, the causative agent of acquired immunodeficiency syndrome (AIDS), are largely unknown. In addition, the molecular mechanism of HIV viral latency is not fully understood. Thus, in order to understand the molecular mechanism of viral latency, we have attempted to clarify the role of cellular miR species that are upregulated in the maintenance of viral latency. Based on two previous reports and the data sets of the upregulated cellular miR upon HIV infection, we searched their target cellular mRNAs using an integrated ontology-based rich-text mining algorithm that enables the target identification of miRs. Using this algorithm we have identified four cellular gene modules namely: module 1 (cell cycle regulators) APOBEC7G, EIF5, MAPK, MYB, MYC, NCOA3, NKFB1, NFKBA1, P300, RAS, RAF, SRC1; module 2 (cell transcription factors) CREBBP, STAT1, STAT5A; module 3 (HIV-1 transcription modulators) RUNX1, TFCP2, YY1, and; module 4 (immune response genes) IFNA 1, IL15, IL6, IL2, IL7, IL10. A high stringency miR target analyses of the 3'-untranslated region (3'-UTR) sequences of these target genes identified common, and multiple in some cases, sequences within these modules. We have deciphered multiple gene regulatory networks using relational software STRINGS, which were consistent with previous observations. The findings clearly demonstrate the cross correlations between miR and mRNA in HIV infected cells.



Ito M, Hamano T, Komatsu T, Asamitsu K, Yamakawa T, Okamoto T.
A novel IKKα inhibitor, noraristeromycin, blocks the chronic inflammation associated with collagen-induced arthritis in mice.
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Tan Gana N. H., Okamoto T.
Interaction between the cellular mRNA and the cellular micro RNA linked to human immunodeficiency virus latency.
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Overlapping glycosylation sequon influences the glycosylation pattern of a chimeric protein expressed in tomato leaf and callus.
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Cueno ME, Imai K, Ochiai K, Okamoto T.
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Asamitsu K, Hibi Y, Imai K, Victoriano AF, Kurimoto E, Kato K, Okamoto T.
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Victoriano AF, Imai K, Togami H, Ueno T, Asamitsu K, Suzuki T, Miyata N, Ochiai K, Okamoto T.
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Tanaka K, Asamitsu K, Uranishi H, Iddamalgoda A, Ito K, Kojima H, Okamoto T.
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Zineldeen DH, Uranishi H, Okamoto T.
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Transgenic Res. 9(5):903-13, 2010

Cueno ME, Hibi Y, Karamatsu K, Yasutomi Y, Imai K, Laurena AC, Okamoto T.
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Ohba K, Ryo A, Dewan MZ, Nishi M, Naito T, Qi X, Inagaki Y, Nagashima Y, Tanaka Y, Okamoto T, Terashima K, Yamamoto N.
Follicular dendritic cells activate HIV-1 replication in monocytes/macrophages through a juxtacrine mechanism mediated by P-selectin glycoprotein ligand 1.
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Imai, K., Ochiai, K, Okamoto, T.
Reactivation of latent HIV-1 infection by the periodontopathic bacterium Porphyromonas gingivalis involves histone modification.
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Futoshi, T , Takahashi, N, Gao N, Akamo Y, Takeyama H, Manabe T and Okamoto T.
Phosphoinositide 3-kinase inhibitor(wortmannin) inhibits pancreatic cancer cell motility and migration induced by hyaluronan in vitro and peritoneal metastatis in vivo.
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